Dr. Ashraf Hossain Talukder

Associate professor

Dept. of Biotechnology and Genetic Engineering

  • Email : ashraf82_bmb@yahoo.com
  • Phone Number : 01818726279

Academic records:  
 
09/2014                             Ph.D.               Tohoku University, Sendai, Japan.

03/2007                             M.S.                 Dhaka University, Dhaka, Bangladesh.

11/2005                             B.Sc. (Hons)    Dhaka University, Dhaka, Bangladesh.
 
Positions:
11/2017 – Present JSPS Post-doctoral fellow, Information Biology Laboratory, Graduate School of Information Sciences (GSIS), Tohoku University, Sendai, Japan. 02/2016 – Present  Associate Professor, Biotechnology and Genetic Engineering Department, Mawlana Bhashani Science and Technology University, Tangail, Bangladesh. 12/2015 – 09/2016 Assistant Professor and Post-doctoral Fellow, Information Biology Laboratory, Graduate School of Information Sciences (GSIS), Tohoku University, Sendai, Japan. 09/2011 – 01/2016 (active or on study leave) Assistant Professor, Biotechnology and Genetic Engineering Department, Mawlana Bhashani Science and Technology University, Tangail, Bangladesh. 10/2011- 09/2014 Ph.D. Student, Information Biology Laboratory, GSIS, Tohoku University, Sendai, Japan. 01/2010 - 08/2011 Lecturer, Biotechnology and Genetic Engineering Department, Mawlana Bhashani Science and Technology University, Tangail, Bangladesh. 06/2009 – 12/2010 Lecturer, Genetic Engineering and Biotechnology Department, Shahjalal Science and Technology University, Sylhet, Bangladesh. 03/2008 – 05/2009 Research Officer, Tuberculosis Laboratory, International Center for Diarrheal Diseases Research, Bangladesh (icddr’b), Dhaka, Bangladesh. 05/2007-  02/2008 Senior Research Assistant, Tuberculosis Laboratory, icddr’b, Dhaka, Bangladesh. 01/2006 – 04/2007 Research Student, Molecular Genetics and Tuberculosis Laboratory, icddr’b, Dhaka, Bangladesh.
 
Peer-reviewed publications:
1. Ushin Fatima, Md. Moniruzzaman, Papia Jahan, Abdullah-Al-Emran, Ashraf Hossain Talukder, and Fatma Ercanli-Huffman. Screening of Significant Risk Factors and Construction of a Score Based Risk Assessment Questionnaire for Earlier Detection of
 
Type 2 Diabetes Mellitus in the Tangail Population. BMC Research Notes, in revision. 2. Ashraf Hossain Talukder, Hasan M. Zahid, and Mamun Rashid Chowdhury. Conjugal transfer of SXT element in Vibrio cholerae recovered from environmental water in Bangladesh. African Journal of Biotechnology, in revision. 3. Gopal Das, Ashraf Hossain Talukder*, Katsuya Uchida, Kazunori Kageyama, and Keiichi Itoi. FosB expression following acute stress in the paraventricular and supraoptic nuclei of the rat hypothalamus. [*Co-first authors] Manuscript under processing. 4. Junko Kono, Kohtarou Konno, Ashraf Hossain Talukder*, Toshimitsu Fuse, Manabu Abe, Katsuya Uchida, Shuhei Horio, Kenji Sakimura, Masahiko Watanabe, and Keiichi Itoi. Distribution of corticotropin-releasing factor neurons in the mouse brain: a study using corticotropin-releasing factor-modified yellow fluorescent protein knock-in mouse. [*Cofirst authors] Brain Structure and Function, May, 2017.   5. Ashraf Hossain Talukder, Shahin Mahmud, Selina Akhter Lira and Md. Abdul Aziz. Phycoremediation of textile effluent by Cyanobacteria (Nostoc muscorum and Anabaena variabilis). Bioresearch Comunication. July, 2015, 1(2), 124-127. 6. Keiichi Itoi, Ashraf Hossain Talukder*, Toshimitsu Fuse, Takuji Kaneko, Ryo Ozawa, Takayuki Sato, Takuma Sugaya, Katsuya Uchida, Maya Yamazaki, Manabu Abe, Rie Natsume, and Kenji Sakimura. Visualization of corticotropin-releasing factor neurons by fluorescent proteins in the mouse brain and characterization of labeled neurons in the paraventricular nucleus of hypothalamus. [*Co-first authors] Endocrinology, October, 2014, 155(10), 4054–4060. 7. Ashraf Hossain Talukder, Hasan M. Zahid and Mamun Rashid Chowdhury. Screening of more selective media of different species of Vibrionaceae family. Journal of Science and Technology, January, 2013, 3(1), 251-259. 8. Nabangshu Shekhar Das, Janmajoy Dey, Dr. Md. Mahbubur Rahman, Hasan M. Zahid, Azizun Nessa Hossain, and Ashraf Hossain Talukder. Bioburden of Human Amniotic Membranes and Inhibition of the Associated Bacteria using Antibiotics and GammaRadiation. Global Journal of Medical Research; Microbiology and Pathology, 2013, 13(2), 1-7. 9. Sayera Banu, Mohammad Khaja Mafij Uddin, Mohammad Riazul Islam, K Zaman,Tahmeed Ahmed, Ashraf Hossain Talukder, Md. Toufiq Rahman, Zeaur Rahim, Nahid Akter, Razia Khatun, Roland Brosch, and Hubert P Endtz. Molecular epidemiology of tuberculosis in rural Matlab of Bangladesh. The international Journal of Tuberculosis and Lung Disease, September, 2011,16(3), 319–326.  10. Md. Al-Emran, M.Azizul Haque, Rokeya Begum, Ashraf Hossain Talukder, M.N. Hossain and Md. Shams-Ud-din. A study on the effects of composite flour on cake quality. Journal of Agricultural Engineering, December, 2010, 38(2), 82-87.
 
Abstracts and Scientific Presentations:                    

1. Ashraf Hossain Talukder, Toshimitsu Fuse, Katsuya Uchida, Naoya Sugimoto, Ryo Ozawa, Maya Yamazaki, Manabu Abe, Rie Natsume, Kenji Sakimura, and Keiichi Itoi. Distribution of fluorescent proteins in the PVN of mouse lines whose CRF neurons are visualized by the fluorescence. Japan Endocrine Society Tohoku Association program, P. 2, 2014. 2. Ashraf Hossain Talukder, Toshimitsu Fuse, Junko Kono, Naoya Sugimoto, Ryo Ozawa, Katsuya Uchida, Maya Yamazaki, Manabu Abe, Rie Natsume, Kenji Sakimura,
 
and Keiichi Itoi. Immunocytochemical characterization of the mouse brain expressing fluorescent proteins selectively in the corticotropin-releasing factor neurons. Program and Abstract s of NIH-Tohoku University- JSPS Symposium, P. 76, 2013. 3. Ashraf Hossain Talukder, Toshimitsu Fuse, Junko Kono, Naoya Sugimoto, Ryo Ozawa, Katsuya Uchida, Maya Yamazaki, Manabu Abe, Rie Natsume, Kenji Sakimura, and Keiichi Itoi. Histological characterization of the mouse brain expressing fluorescent proteins selectively in the corticotropin-releasing factor neurons. Program and Abstracts of the 36th Annual meeting of Japan Neuroscience Society, P. 258, 2013. 4. Ashraf Hossain Talukder, Toshimitsu Fuse, Katsuya Uchida, Naoya Sugimoto, Ryo Ozawa, Maya Yamazaki, Manabu Abe, Rie Natsume, Kenji Sakimura, and Keiichi Itoi. Distribution of fluorescent proteins in the PVN of mouse lines whose CRF neurons are visualized by the fluorescence. Endocrine Magazine, 89, 596, 2013.                                                                          Professional Memberships:
201 2- Present Neuroendocrine Society, Japan. 2012 - Present Japan Neuroscience Society (JNS), Japan. 2006 - Present Bangladesh Society for Biochemistry and Molecular Biology (BSBMB) 2006 - Present Graduate Biochemist’s Association (GBA), Bangladesh.
 
Honors and Awards:
11/2017- 10/2019 JSPS Post – doctoral fellowship, Japan Government, Japan. 10/2011- 09/2014 Monbukagakusho Scholarship, Japan Government, Japan. 2003  Dean’s Honour, Faculty of Biological Sciences, University of Dhaka.
 
Technical Experiences:
In vivo experiment by employing animal model:  Mice: Bilateral adrenalectomy, icv microinjection, Microinjection to PVH and LC, Transcardial perfusion, Blood collection from different system, Brain Separation and fixation.  Rat: Bilateral adrenalectomy, Transcardial perfusion, Brain Separation and fixation. Biochemistry, Immunology, Molecular Biology:  Immunohistochemistry: Free floating, fixed, ABC system, TSA system.  In-situ hybridization: using radio- and fluorescence-labeled probes, TSA system.   Protein Chemistry: Western blotting, ELISA.  Manipulation of DNA (Molecular cloning, DNA sequencing, Isolation of plasmid, chromosomal DNA, bacteriophage DNA, and phage particle)   Manipulation of RNA and DNA (Isolation of RNA and DNA, preparation of cDNA to analyze gene expression using RT-PCR, quantitative RT-PCR, Southern blotting using radio- and fluorescence-labeled probes, Spoligotyping, MIRU-VNTR).   Transformation, transduction and conjugation of plasmids or transposable elements into bacteria (i.e., Vibrio cholerae, E. coli, etc.)  In silico approaches   Cell Counter, Intensity analysis by Image J.  Optical fractionator, MBF Bioscience.
 
 Homology search, Multiple sequence alignment, Sequence conservation analysis, Analysis of transcription binding site, Protein post-translational modification analysis, Primer designing.
 
 
Personal Statement:
 
In 2007, I completed my thesis work in the Molecular Genetics Laboratory of International Center for Diarrheal Diseases Research, Bangladesh (icddr’b) and studied on “Distribution of SXT element in Vibrio species isolated under stress condition from environmental Water”. In that study I screened selective culture media for each Vibrio species by using their salt tolerant properties and transfer of SXT element. The overall bacterial growth was markedly decreased at high NaCl concentration, but Vibrio fluvialis, Vibrio mimicus and Aeromonas sp were more salt-tolerant than other species of Vibrionaceae family. The SXT element could be transferred from Vibrio cholerae O1 to other V. cholerae strains (Talukder et al., Journal of Science and Technology, 2013).
 
As a research officer in the Tuberculosis Laboratory of icddr’b,  I had conducted my study on “Molecular epidemiology of tuberculosis in rural Matlab, Bangladesh”. The study focused on the characterization and classification of clinically isolated tuberculosis (TB) bacteria from patients in rural Bangladesh (Banu, Talukder et al., The International Journal of Tuberculosis and Lung Disease, 2012).
 
In 2011, I was awarded the most prestigious and competitive Japanese Government scholarship to pursue my post-graduate study under the supervision of Prof. Keiichi Itoi of Tohoku University, Sendai, Japan. During my Ph.D. study I worked on “Visualization of corticotropin-releasing factor neurons by fluorescent proteins in the mouse brain and characterization of labeled neurons in the paraventricular nucleus of hypothalamus”. In this study we described and characterized two mouse line that generated for the purpose of visualizing CRF neurons and elucidating cellular and extracellular substrates for regulating CRF neurons. It was also demonstrated that noradrenaline (NA), a major neurotransmitter in the PVN, increases the frequency of excitatory post-synaptic currents in the PVN-Venus neurons (Itoi*, Talukder*, et al., Endocrinology, 2014, *, equally contributing authors).                In 2015-2016, as an Assistant Professor in the same laboratory I also characterized another noble mouse line CRF-VenusNeo mouse and studied the morphologic, topographic, and cytoarchitectonic features of Venus-expressing neurons in the mouse brain. In this study, which utilized Venus as a marker for CRF neurons, the overall distribution of CRF neurons in the mouse brain was similar to that in the rat brain and also demonstrated that the CRF neurons contain glutamate in the Piriform cortex and Inferior olive, while they contain gamma-aminobutyric acid in the neocortex, the bed nucleus of the stria terminalis, the hippocampus, and the amygdala and also demonstrated to be cholinergic in the midbrain tegmentum (Kono*, Konno*, Talukder*, et al., Brain Structure and Function, 2016, *, equally contributing authors).
 
Given my long term research interest in different field I believe I can make a significant contribution to a thriving research group.